ChIP-Seq Profiling of Estrogen Receptor Alpha Binding Sites Using the Illumina Genome Analyzer
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چکیده
Regulation of gene expression is a key event during development, differentiation, signaling, and adaptation to environmental cues. Gene expression is regulated at many levels. Histone modifications and binding of transcription factors to their cognate target sites in promoters and enhancers lead to the recruitment of activating or repressing cofactors and ultimately in alteration of mRNA levels. To decipher the network of target genes governed by transcription factors, it is essential to identify their genomic binding site repertoire.
منابع مشابه
ILLUMINA SEQUENCING Whole-Genome Chromatin IP Sequencing (ChIP-Seq) Illumina ChIP-Seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify binding sites of DNA-associated proteins. Illumina ChIP-Seq technology precisely and cost-effectively maps global binding sites for a protein of interest
INTRODUCTION Transcription factors and other chromatin-associated proteins are essential phenotype-influencing mechanisms. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states. This epigenetic information is complimentary to genotype and expression analysis. Traditional methods have successfully ...
متن کاملILLUMINA SEQUENCING Whole-Genome Chromatin IP Sequencing (ChIP-Seq) Illumina ChIP-Seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify binding sites of DNA-associated proteins. Illumina ChIP-Seq technology precisely and cost-effectively maps global binding sites for a protein of interest
INTRODUCTION Transcription factors and other chromatin-associated proteins are essential phenotype-influencing mechanisms. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states. This epigenetic information is complimentary to genotype and expression analysis. Traditional methods have successfully ...
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تاریخ انتشار 2014